Shanghai Model Organisms Center,Inc
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    Overexpression Model - Rosa26 Knock In

    2014/12/18 Viewers:

    Rosa26 Knock In
    ROSA26 is a locus widely used for constitutive, ubiquitous gene expression in mice. The gene of interest is targeted into the first intron of the locus. The expression of the gene can be driven by endogenous ROSA26 promoter or by a specific promoter.



    Workflow via CRISPR/Cas9:


    Workflow via ES targeting: